Analysis of immunization with DNA encoding Pseudomonas aeruginosa exotoxin A

Use of immunogenic moiety of Pseudomonas aeruginosa exotoxin A as a DNA vaccine in experimentally contaminated mice

Background: DNA immunization is an appropriate method to produce an immunological response. Pseudomonas aeruginosa produces exotoxin A which is highly cytotoxic for eukaryotic cells. Since domains II (translocation domain) and 1b of the toxin have antigenic qualities, so they could be  useful candidates to protect against pseudomonas infections. Objectives: To evaluate if recombinant plasmid co...

Protection against experimental Pseudomonas aeruginosa infection in mice by active immunization with exotoxin A toxoids.

The immunoprophylactic effect of chemically inactivated Pseudomonas aeruginosa exotoxin A in experimental pseudomonas infections was studied. Exotoxin A toxoids were prepared by Formalin (f-TXD) or glutaraldehyde (g-TXD) treatment. Immunization of mice with three or four doses (10 micrograms each) of f-TXD and the synthetic adjuvant N-acetylmuramyl-L-alanyl-D-isoglutamine (50 micrograms) induce...

PREPARATION AND EVALUATION OF PROPERTIES OF PSEUDOMONAS AERUGINOSA EXOTOXIN A CONJUGATE WITH GOLD NANOPARTICLES

Background & Aims: Pseudomonas aeruginosa is an opportunistic pathogen and most important cause of hospital infections, which causes septicemia and death through various virulence factors such as exotoxin A. In the present study, P. aeruginosa exotoxin A (as a toxic factor) was prepared with the aim of obtaining a new recombinant vaccine candidate and conjugated with gold nanoparticles and its ...

Purification and characterization of Pseudomonas aeruginosa exotoxin.

A trypsin-sensitive, heat-labile exotoxin of Pseudomonas aeruginosa strain P-A-103 has been purified by a procedure that includes membrane ultrafiltration, hydroxylapatite chromatography, ion-exchange cellulose chromatography, and gel filtration chromatography. The procedure resulted in the recovery of 48% of the exotoxin with a 40-fold increase in specific activity (micrograms of protein per m...

Pseudomonas aeruginosa exotoxin: effect on cell cultures.